Composite

Part:BBa_K2638017:Design

Designed by: Erika Schneider   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


Weak constitutive expression system for HmtA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 978
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
    Illegal NgoMIV site found at 812
    Illegal NgoMIV site found at 934
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1349
    Illegal NgoMIV site found at 1670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 407


Design Notes

Biobrick Assembly by restriction and ligation.


Source

Genome of Pseudomonas brassicacearum 3Re2-7 13 and iGEM Distribution Plates from 2018

References